WHEN YOU NEED TO USE DIKE?

During a biological experiment to detect an affinity reaction, a molecule of interest is attached to the quartz crystal on the measure channel, this attachment can be obtained through direct coupling or capture by another protein, the result of this process is that to make the quartz crystal sensitive to the molecule to be detected.

After this phase a suitable number of actions is performed on the biological sample to analize so that the affinity reaction can take place, some of these operation can involve temperature changes, but the quartz crystal response is very sensitive to temperature, so it is very difficult to separate the response to the temperature change from that due to the affinity reaction, and often it is not possible to work at constant temperature especially when you work with biological samples.

Dike can overcame this problem because it has two input channels to work with two different quartz crystals: the molecule of interest is immobilized onto a crystal quartz that is used as measure channel, while the other crystal quartz on the second channel is used without the immobilized molecule of interest, so it acts as reference channel.

The measure channel is sensitive to the temperature changes and to the affinity reaction, while the reference channel is sensitive only to the temperature changes because the affinity reaction can't take place since on its surface is not present the immobilized molecule of interest.

With this approach you can reveal the affinity reaction simply subtracting the response of the measure channel from that of the reference channel, because both channel have the same response for temperature changes while only the mesure channel is sensitive to the affinity reaction.

This differential approach uses two channels to detect the affinity reaction and to eliminate the response of the quartz crystal to all the variations of the experiment conditions.